Saprospira grandis Medium

[所属分类:培养基配方] [发布时间:2021-8-11] [发布人:网站管理员2] [阅读次数:] [返回]

Saprospira grandis Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
Ca(NO 3 ) 2 ·4H 2 O ............................................................................0.1g
K 2 HPO 4 .......................................................................................0.02g
Seawater, filtered..........................................................................1.0L
Trace elements.........................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements:
Composition per liter:
FeSO 4 ·7H 2 O...............................................................................0.5mg
ZnSO 4 ·7H 2 O..............................................................................0.3mg
H 3 BO 3 ........................................................................................0.1mg
CoCl 2 ·6H 2 O ...............................................................................0.1mg
CuSO 4 ·5H 2 O..............................................................................0.1mg
MnSO 4 ·4H 2 O.............................................................................0.1mg
Na 2 MoO 4 ·2H 2 O .........................................................................0.1mg
Preparation of Trace Elements: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Combine components. Mix thoroughly.
Adjust pH to 7.0. Filter sterilize.
Use: For the cultivation of Saprospira grandis.