SAP 2 Agar

[所属分类:培养基配方] [发布时间:2021-8-11] [发布人:网站管理员2] [阅读次数:] [返回]

SAP 2 Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Pancreatic digest of casein............................................................1.0g
Yeast extract..................................................................................1.0g
Artificial seawater.........................................................................1.0L
pH 7.2 ± 0.2 at 25°C
Artificial Seawater:
Composition per liter:
NaCl............................................................................................24.7g
MgSO 4 ·7H 2 O................................................................................6.3g
MgCl 2 ·6H 2 O .................................................................................4.6g
CaCl 2 .............................................................................................1.0g
KCl................................................................................................0.7g
NaHCO 3 ........................................................................................0.2g
Preparation of Artificial Seawater: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add solid components to 1.0L of artifi-
cial seawater. Mix thoroughly. Gently heat and bring to boiling. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and cultivation of Cytophaga species, Herpeto-
siphon species, Saprospira species, and Flexithrix species.