Salmonella Shigella Agar, Modified (SS Agar, Modified)

[所属分类:培养基配方] [发布时间:2021-8-10] [发布人:网站管理员2] [阅读次数:] [返回]

Salmonella Shigella Agar, Modified
(SS Agar, Modified)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................12.0g
Lactose........................................................................................10.0g
Sodium citrate.............................................................................10.0g
Na 2 S 2 O 3 ........................................................................................8.5g
Bile salts........................................................................................5.5g
Beef extract...................................................................................5.0g
Peptone..........................................................................................5.0g
Ferric citrate..................................................................................1.0g
Neutral Red...............................................................................0.025g
Brilliant Green .........................................................................0.33mg
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Do not autoclave. Cool to 45°–50°C. Pour
into sterile Petri dishes in 20.0mL volumes. Allow the surface of the
plates to dry before inoculation.
Use: For the selective isolation and differentiation of pathogenic
enteric bacilli, especially those belonging to the genus Salmonella.
This medium provides better growth of Shigella species. Lactose-fer-
menting bacteria such as Escherichia coli or Klebsiella pneumoniae
appear as small pink or red colonies. Lactose-nonfermenting bacte-
ria—such as Salmonella species, Proteus species, and Shigella spe-
cies—appear as colorless colonies. Production of H 2 S by Salmonella
species turns the center of the colonies black.