Salmonella Shigella Agar (SS Agar)

[所属分类:培养基配方] [发布时间:2021-8-10] [发布人:网站管理员2] [阅读次数:] [返回]

Salmonella Shigella Agar
(SS Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................13.5g
Lactose........................................................................................10.0g
Bile salts........................................................................................8.5g
Na 2 S 2 O 3 ........................................................................................8.5g
Sodium citrate...............................................................................8.5g
Beef extract...................................................................................5.0g
Pancreatic digest of casein............................................................2.5g
Peptic digest of animal tissue .......................................................2.5g
Ferric citrate..................................................................................1.0g
Neutral Red...............................................................................0.025g
Brilliant Green.........................................................................0.33mg
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Do not autoclave. Cool to 45°–50°C. Pour
into sterile Petri dishes in 20.0mL volumes. Allow the surface of the
plates to dry before inoculation.
Use: For the selective isolation and differentiation of pathogenic
enteric bacilli, especially those belonging to the genus Salmonella.
This medium is not recommended for the primary isolation of Shigella
species. Lactose-fermenting bacteria such as Escherichia coli or Kleb-
siella pneumoniae appear as small pink or red colonies. Lactose-non-
fermenting bacteria—such as Salmonella species, Proteus species, and
Shigella species—appear as colorless colonies. Production of H 2 S by
Salmonella species turns the center of the colonies black.