Saline Czapek Agar (SCZA)

[所属分类:培养基配方] [发布时间:2021-8-10] [发布人:网站管理员2] [阅读次数:] [返回]

Saline Czapek Agar
(SCZA)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................30.0g
NaCl............................................................................................25.0g
Agar............................................................................................15.0g
NaNO 3 ..........................................................................................3.0g
K 2 HPO 4 .........................................................................................1.0g
KCl................................................................................................0.5g
MgSO 4 ·7H 2 0.................................................................................0.5g
FeSO 4 ·7H 2 O................................................................................0.01g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except sucrose, to dis-
tilled/deionized water and bring volume to 900.0mL. Mix thoroughly.
Distribute into tubes or flasks. In a separate flask, add sucrose to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave both solutions separately for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Combine the sterile solutions. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.