Sakazakii DHL Agar

[所属分类:培养基配方] [发布时间:2021-8-10] [发布人:网站管理员2] [阅读次数:] [返回]

Sakazakii DHL Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Casein enzymic hydrolysate .......................................................10.0g
Meat peptone ..............................................................................10.0g
Lactose........................................................................................10.0g
Sucrose........................................................................................10.0g
Meat extract..................................................................................3.0g
Na 2 S 2 O 3 ........................................................................................2.0g
Sodium deoxycholate....................................................................1.5g
Sodium citrate...............................................................................1.0g
Ammonium iron (III) citrate.........................................................1.0g
L -Cysteine·HCl·H 2 O.....................................................................0.2g
Neutral Red.................................................................................0.03g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the detection and isolation of pathogenic Enterobacteriaceae
from all types of specimens.