Sabouraud Glucose Agar, HiVeg

[所属分类:培养基配方] [发布时间:2021-8-9] [发布人:网站管理员2] [阅读次数:] [返回]

Sabouraud Glucose Agar, HiVeg

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................40.0g
Agar ............................................................................................15.0g
Plant peptone No. 4.....................................................................10.0g
Selective supplement...............................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Selective Supplement:
Composition per 10.0mL:
Cycloheximide..............................................................................0.5g
Chloramphenicol.........................................................................0.04g
Preparation of Selective Supplement: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Caution: Cycloheximide is very toxic. Avoid skin contact or aerosol
formation and inhalation.
Preparation of Medium: Add components, except selective sup-
plement, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically add 10.0mL sterile selective supplement. Mix thor-
oughly. Pour into sterile Petri dishes or aseptically distribute into tubes.
Use: For the cultivation of dermatophytes and other pathogenic and
nonpathogenic fungi from clinical and nonclinical specimens. For the
cultivation of yeast and filamentous fungi.