Sabouraud Glucose Agar with Chloramphenicol and Cycloheximide

[所属分类:培养基配方] [发布时间:2021-8-9] [发布人:网站管理员2] [阅读次数:] [返回]

Sabouraud Glucose Agar with
Chloramphenicol and Cycloheximide

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................40.0g
Agar ............................................................................................15.0g
Pancreatic digest of casein............................................................5.0g
Peptic digest of animal tissue........................................................5.0g
Cycloheximide solution...........................................................10.0mL
Chloramphenicol solution........................................................10.0mL
pH 5.6 ± 0.2 at 25°C
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide..............................................................................0.5g
Acetone....................................................................................10.0mL
Preparation of Cycloheximide Solution: Add cycloheximide to
acetone. Mix thoroughly.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Chloramphenicol Solution:
Composition per 10.0mL:
Chloramphenicol.........................................................................0.05g
Ethanol (95% solution)............................................................10.0mL
Preparation of Chloramphenicol Solution: Add chlorampheni-
col to ethanol. Mix thoroughly.
Preparation of Medium: Add components, except cycloheximide
solution and chloramphenicol solution, to distilled/deionized water and
bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to
boiling. Add the cycloheximide solution and chloramphenicol solu-
tion. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in
tubes.
Use: For the cultivation and identification of yeasts.