SABHI HiVeg Agar Base with Blood and Chloromycetin

[所属分类:培养基配方] [发布时间:2021-8-9] [发布人:网站管理员2] [阅读次数:] [返回]

SABHI HiVeg Agar Base with Blood and
Chloromycetin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................21.0g
Agar............................................................................................15.0g
Plant infusion..............................................................................5.14g
Plant peptone No. 3.......................................................................5.0g
Plant special peptone ....................................................................5.0g
Plant special infusion.................................................................. 4.11g
NaCl..............................................................................................2.5g
Na 2 HPO 4 .....................................................................................1.25g
Blood......................................................................................100.0mL
Chloromycetin solution .............................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without blood and chloromycetin, is available
as a premixed powder from HiMedia.
Chloromycetin Solution:
Composition per 10.0mL:
Chloromycetin..............................................................................1.0g
Preparation of Chloromycetin Solution: Add chloromycetin to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except blood and chloro-
mycetin solution, to distilled/deionized water and bring volume to
899.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
100.0mL of sterile blood and 1.0mL of sterile chloromycetin solution.
Sheep blood or human blood may be used. Mix thoroughly. Aseptically
distribute into sterile tubes in 5.0mL volumes.
Use:  For the cultivation of dermatophytes and other pathogenic and
nonpathogenic fungi from clinical and nonclinical specimens. Blood
enhances the recovery of Blastomyces dermatitidis and Histoplasma
capsulatum and their conversion to the yeast phase.