SABHI HiVeg Agar Base with Chloromycetin

[所属分类:培养基配方] [发布时间:2021-8-9] [发布人:网站管理员2] [阅读次数:] [返回]

SABHI HiVeg Agar Base with Chloromycetin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................21.0g
Agar ............................................................................................15.0g
Plant infusion..............................................................................5.14g
Plant peptone No. 3.......................................................................5.0g
Plant special peptone ....................................................................5.0g
Plant special infusion..................................................................4.11g
NaCl..............................................................................................2.5g
Na 2 HPO 4 .....................................................................................1.25g
Chloromycetin solution..............................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without chloromycetin, is available as a pre-
mixed powder from HiMedia.
Chloromycetin Solution:
Composition per 10.0mL:
Chloromycetin ..............................................................................1.0g
Preparation of Chloromycetin Solution: Add chloromycetin to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except chloromycetin
solution, to distilled/deionized water and bring volume to 999.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 1.0mL of
sterile chloromycetin solution. Mix thoroughly. Aseptically distribute
into sterile tubes in 5.0mL volumes.
Use:  For the cultivation of dermatophytes and other pathogenic and
nonpathogenic fungi from clinical and nonclinical specimens.