Rhodovulum strictum Medium (DSMZ Medium 746)

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodovulum strictum Medium
(DSMZ Medium 746)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution 1...............................................................................500.0mL
Solution 2...............................................................................500.0mL
pH 7.8 ± 0.2 at 25°C
Solution 1:
Composition per 500.0mL:
NaCl..............................................................................................8.2g
Na- DL -malate................................................................................3.6g
Yeast extract..................................................................................1.0g
(NH 4 ) 2 SO 4 ....................................................................................1.0g
Na2 S 2O 3 ·5H 2 O..............................................................................0.5g
MgCl 2 ·6H 2 O .................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.05g
Trace elements solution SL-8....................................................1.0mL
Trace Elements Solution SL-8:
Composition per liter:
Na 2 -EDTA ....................................................................................5.2g
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O...........................................................................190.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
H 3 BO 3 ......................................................................................62.0mg
Na 2 MoSO 4 ·2H 2 O.....................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
CuCl 2 ·2H 2 O.............................................................................17.0mg
Preparation of Trace Elements Solution SL-8: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Sparge with 100% N 2 .
Preparation of Solution 1 : Prepare and dispense medium under an
oxygen-free 100% N 2 . Add components to distilled/deionized water
and bring volume to 500.0L. Mix thoroughly. Sparge with 100% N 2 .
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Solution 2:
Composition per 500mL:
K 2 HPO 4 .......................................................................................1.35g
KH 2 PO 4 .......................................................................................0.35g
Preparation of Solution 2:  Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 7.8.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper-
ature.
Preparation of Medium: Aseptically and anaerobically combine
500.0mL solution 1 and 500.0mL solution 2 under N 2 . Mix thoroughly.
Adjust pH to 7.8. Aseptically and anaerobically distribute into sterile
screw-cap tubes or flasks.
Use: For the cultivation of Rhodovulum strictum.