Rhodovulum kholense Medium (DSMZ Medium 1136)

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodovulum kholense Medium
(DSMZ Medium 1136)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ...........................................................................................20.0g
Sodium pyruvate ..........................................................................3.0g
MgSO 4 ·7H 2 O................................................................................2.0g
NH 4 Cl ........................................................................................0.64g
KH 2 PO 4 ........................................................................................0.5g
Yeast extract .................................................................................0.5g
CaC l 2 ·2H 2 O ................................................................................0.12g
Trace elements solution SL-12 ..................................................1.0mL
pH 5.7 ± 0.2 at 25°C
Trace Elements Solution SL-12:
Composition per liter:
FeSO 4 ·7H 2 O..................................................................................1.1g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ................................................................................0.19g
MnCl 2 ·2H 2 O................................................................................0.05g
ZnCl 2 ........................................................................................42.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
Na 2 MoO 4 ·4H 2 O .......................................................................18.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
Preparation of Trace Elements Solution SL-12:  Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Distribute into tubes or flasks. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Rhodoblastus spp.