Rhodospirillum salinarum Medium

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodospirillum salinarum Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................100.0g
KCl................................................................................................5.0g
MgCl 2 ·6H 2 O .................................................................................5.0g
MgSO 4 ·7H 2 O................................................................................5.0g
NH 4 ·Cl ..........................................................................................5.0g
Peptone solution.......................................................................30.0mL
Yeast extract solution...............................................................30.0mL
Ferric citrate solution...............................................................10.0mL
Trace elements solution.............................................................5.0mL
Peptone Solution:
Composition per 100.0mL:
Peptone .......................................................................................15.0g
Preparation of Peptone Solution:  Add peptone to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract................................................................................15.0g
Preparation of Yeast Extract Solution:  Add yeast extract to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Ferric Citrate Solution:
Composition per 10.0mL:
Ferric citrate..................................................................................0.1g
Preparation of Ferric Citrate Solution:  Add ferric citrate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Trace Elements Solution:
Composition per liter:
CoCl 2 ·6H 2 O ................................................................................10.0g
ZnSO 4 ·7H 2 O..........................................................................220.0mg
MgCl 2 ·4H 2 O...........................................................................180.0mg
Na 2 MoO 4 ·2H 2 O .........................................................................6.3mg
CuSO 4 ·5H 2 O..............................................................................1.0mg
Preparation of Medium: Add components, except peptone solu-
tion, yeast extract solution, ferric citrate solution, and trace elements
solution to distilled/deionized water and bring volume to 925.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Asep-
tically add 30.0mL of sterile peptone solution, 30.0mL of sterile yeast
extract solution, 10.0mL of sterile ferric citrate solution, and 5.0mL of
sterile trace elements solution. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of Rhodospirillum salinarum.