Rhodospirillum Medium, Modified IV

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodospirillum Medium, Modified IV

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................25.0g
Yeast extract..................................................................................1.0g
Disodium succinate.......................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.4g
NaCl..............................................................................................0.4g
NH 4 Cl ...........................................................................................0.4g
CaCl 2 ·2H 2 O ................................................................................0.05g
Ferric citrate (0.1% solution).....................................................5.0mL
Trace elements solution SL-6....................................................1.0mL
Ethanol.......................................................................................0.5mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O ...............................................................................0.03g
Na 2 MoO 4 ·H 2 O............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 · 2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 3.4.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8. Dis-
tribute 40.0mL volumes into tubes or bottles. Sparge with 100% N 2 for
1–2 min. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Rhodobacter sulfidophilus.