Rhizobium japonicum Agar

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhizobium japonicum Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Mannitol......................................................................................10.0g
Yeast extract..................................................................................1.0g
Soil extract.............................................................................200.0mL
Soil Extract:
Composition per liter:
African Violet soil.......................................................................77.0g
Na 2 CO 3  .........................................................................................0.2g
Preparation of Soil Extract: Add components to 1.0L of tap water.
Autoclave for 15 min at 15 psi pressure–121°C. Filter through What-
man filter paper. Bring volume to 1.0L with tap water.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Bradyrhizobium japoni-
cum.