Rhizobium BIII Defined Broth

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhizobium BIII Defined Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Mannitol......................................................................................10.0g
Sodium glutamate.........................................................................1.1g
K 2 HPO 4 .......................................................................................0.23g
MgSO 4 ·7H 2 O................................................................................0.1g
Trace elements stock..................................................................1.0mL
Vitamin stock.............................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Stock:
Composition per liter:
Nitrilotriacetic acid.......................................................................7.0g
CaCl 2 ·2H 2 O.................................................................................6.62g
H 3 BO 3 .......................................................................................0.145g
FeSO 4 ·7H 2 O..............................................................................0.125g
Na 2 MoO 4 ...................................................................................0.125g
ZnSO 4 ·7H 2 O.............................................................................0.108g
CoSO 4 ·7H 2 O...............................................................................0.07g
CuSO 4 ·5H 2 O..............................................................................5.0mg
MnCl 2 ·4H 2 O...............................................................................4.3mg
Preparation of Trace Elements Stock: Add components, except
nitrilotriacetic acid, to 500.0mL of distilled/deionized water in the or-
der listed. Adjust pH to 5.0. Add nitrilotriacetic acid. Bring volume to
1.0L with distilled/deionized water.
Vitamin Stock:
Composition per liter:
Inositol........................................................................................0.12g
p-Aminobenzoic acid..................................................................0.02g
Biotin ..........................................................................................0.02g
Calcium pantothenate .................................................................0.02g
Nicotinic acid..............................................................................0.02g
Pyridoxine·HCl...........................................................................0.02g
Riboflavin ...................................................................................0.02g
Thiamine·HCl .............................................................................0.02g
Sodium phosphate buffer (50.0mM solution, pH 7.0)..................1.0L
Preparation of Vitamin Stock: Combine components. Mix thor-
oughly. Filter sterilize. Store at 4°C in the dark.
Preparation of Medium: Add components, except vitamin stock,
to distilled/deionized water and bring volume to 999.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically add 1.0mL of sterile vitamin stock. Mix thoroughly. Asep-
tically distribute into sterile tubes or flasks.
Use:  For the isolation and cultivation of Rhizobium species.