RF Medium

[所属分类:培养基配方] [发布时间:2021-8-3] [发布人:网站管理员2] [阅读次数:] [返回]

RF Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................0.05g
Peptone .......................................................................................0.05g
(NH 4 ) 2 SO 4 ..................................................................................0.05g
L -Cysteine·HCl·H 2 O...................................................................0.05g
Salt solution.............................................................................50.0mL
Rumen fluid, clarified..............................................................30.0mL
Resazurin (1% solution) ............................................................0.1mL
pH 7.4 ± 0.2 at 25°C
Salt Solution:
Composition per liter:
NaHCO 3 ......................................................................................10.0g
NaCl..............................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
CaCl 2 , anhydrous..........................................................................0.2g
MgSO 4 ..........................................................................................0.2g
Preparation of Salts Solution: Add CaCl 2 and MgSO 4 to 300.0mL
of distilled/deionized water. Mix thoroughly until dissolved. Bring vol-
ume to 800.0mL with distilled/deionized water. Add remaining com-
ponents while stirring. Bring volume to 1.0L. Mix thoroughly. Store at
4°C.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.2–6.3
with 4N HCl. Gently heat and bring to boiling under 100% N 2 . Anaer-
obically distribute into tubes in 7.0mL volumes. Cap with rubber stop-
pers. Place tubes in a press. Autoclave for 20 min at 15 psi pressure–
121°C with fast exhaust. The pH of the medium should be 7.4 after au-
toclaving.
Use:  For the cultivation and maintenance of Treponema bryantii.