Reduced Transport Fluid

[所属分类:培养基配方] [发布时间:2021-8-3] [发布人:网站管理员2] [阅读次数:] [返回]

Reduced Transport Fluid

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Stock mineral salt solution No. 1.............................................75.0mL
Stock mineral salt solution No. 2.............................................75.0mL
Dithiothreitol (1% solution).....................................................20.0mL
Ethylenediamine tetraacetic acid
(1M solution).....................................................................10.0mL
Na 2 CO 3  (8% solution)...............................................................5.0mL
Resazurin (0.1% solution)..........................................................1.0mL
pH 8.0 ± 0.2 at 25°C
Stock Mineral Salt Solution No. 1:
Composition per 100.0mL:
K 2 HPO 4 .........................................................................................0.6g
Preparation of Stock Mineral Salt Solution No. 1: Add
K 2 HPO 4 to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly.
Stock Mineral Salt Solution No. 2:
Composition per 100.0mL:
NaCl..............................................................................................1.2g
(NH 4 ) 2 SO 4 .....................................................................................1.2g
K 2 HPO 4 .........................................................................................0.6g
MgSO 4 ·7H 2 O..............................................................................0.25g
Preparation of Stock Mineral Salt Solution No. 2: Add com-
ponents to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Asep-
tically distribute into sterile tubes with rubber stoppers.
Use: For the transport and isolation of bacteria from dental plaque,
especially Streptococcus mutans and Streptococcus sanguis. Also used
for the cultivation of a variety of Gram-positive bacteria from the oral
cavity, especially streptococci, actinomycetes, lactobacilli, clostrida,
Bacteroides, Fusobacteria, and Veillonela.