Reduced Transport Fluid

[所属分类:培养基配方] [发布时间:2021-8-3] [发布人:网站管理员2] [阅读次数:] [返回]

Reduced Transport Fluid

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 .....................................................................................9.0g
NaCl..............................................................................................9.0g
K 2 HPO 4 .........................................................................................4.5g
KH 2 PO 4 .........................................................................................4.5g
Na 2 CO 3  .........................................................................................4.0g
EDTA (ethylenediamine tetraacetic acid).....................................3.8g
Dithiothreitol.................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................1.8g
pH 8.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Asep-
tically distribute into sterile tubes with rubber stoppers.
Use: For the transport and isolation of bacteria from dental plaque,
especially Streptococcus mutans and Streptococcus sanguis. Also used
for the cultivation of a variety of Gram-positive bacteria from the oral
cavity, especially streptococci, actinomycetes, lactobacilli, clostridia,
Bacteroides species, Fusobacterium species, and Veillonella species.