Alkvisco Medium

[所属分类:培养基配方] [发布时间:2019-9-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkvisco Medium
Composition per liter:
Agar ............................................................................................15.0g
Beef extract.................................................................................10.0g
Peptone........................................................................................10.0g
NaCl..............................................................................................5.0g
Acrylonitrile..................................................................................0.5g
KCN.........................................................................................10.0mg
pH 6.5–8.0 at 25°C
Caution: Cyanide is toxic. Acrylonitrile is a carcinogen; use appro-
priate precautions.
Preparation of Medium: Add components, except acrylonitrile, to
distilled/deionized water and bring volume to 980.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 10 min at 15 psi
pressure–121°C. Add acrylonitrile to 20.0mL of distilled/deionized
water and filter sterilize. Add aseptically to the sterile basal medium.
Use: For the cultivation and maintenance of Bacillus subtilis and
Corynebacterium species.