Alkalophilic Halophile Broth

[所属分类:培养基配方] [发布时间:2019-9-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkalophilic Halophile Broth
Composition per liter:
Solution A..............................................................................500.0mL
Solution B..............................................................................500.0mL
pH 9.5 ± 1.0 at 25°C
Solution A:
Composition per 500.0mL:
NaCl..........................................................................................200.0g
Na 2 CO 3 ·10H 2 O...........................................................................50.0g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution B:
Composition per liter:
Yeast extract................................................................................10.0g
Casamino acids.............................................................................7.5g
Trisodium citrate...........................................................................3.0g
KCl................................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................1.0g
FeSO 4 ·7H 2 O.............................................................................50.0mg
MnCl 2 ·4H 2 O.............................................................................0.36mg
Preparation of Solution B: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Preparation of Medium: Aseptically combine 500.0mL of solu-
tion A with 500.0mL of solution B. Mix thoroughly. Bring pH to 9.5.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Natronobacterium gregoryi, Natronobac-
terium magadii, Natronobacterium pharaonis, Natronobacterium vac-
uolata, and Natronococcus occultus.