Alkaline Nutrient Agar

[所属分类:培养基配方] [发布时间:2019-9-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkaline Nutrient Agar
Composition per liter:
Agar............................................................................................15.0g
Peptone .........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Beef extract...................................................................................1.0g
Sodium sesquicarbonate solution ..........................................100.0mL
pH 9.7 ± 0.2 at 25°C
Sodium Sesquicarbonate Solution:
Composition per 100.0mL:
Na 2 CO 3 , anhydrous.....................................................................10.6g
NaHCO 3 ......................................................................................8.42g
Preparation of Sodium Sesquicarbonate Solution : Add com-
ponents to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize. Warm to 50°–55°C.
Preparation of Medium: Add components, except sodium sesquicar-
bonate solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add sterile sodium
sesquicarbonate solution. Mix thoroughly. Adjust pH to 9.7. Pour into ster-
ile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of alkaliniphilic bacteria, including Bacillus
alcalophilus, Bacillus cohnii, and other Bacillus species.