NTYG

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

NTYG

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................10.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
Fetal bovine serum, dialyzed...................................................20.0mL
Sheep blood, defibrinated........................................................10.0mL
Fetal Bovine Serum, Dialyzed:
Composition per 100.0mL:
Fetal bovine serum, heat inactivated......................................100.0mL
Preparation of Fetal Bovine Serum, Dialyzed: Dialyze the
heat-inactivated serum at 0–4°C against 10 volumes of distilled/deion-
ized water. Clean the dialysis tubing before use by boiling in 1.0L of a
0.037% EDTA solution. Rinse the tubing with distilled/deionized wa-
ter. Change the water four times at 8–16 hr intervals. Centrifuge the di-
alyzed serum for 30 min at 35,000X g. Filter sterilize.
Preparation of Medium: Add components, except dialyzed fetal
bovine serum and sheep blood, to distilled/deionized water and bring
volume to 975.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 20.0mL of sterile, dialyzed fetal bo-
vine serum, and 5.0mL of sterile sheep blood. Mix thoroughly. Asepti-
cally distribute into sterile screw-capped tubes or flasks.
Use: For the cultivation of Naegleria lovaniensis.