Nitrosomonas Medium

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrosomonas Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 ....................................................................................3.0g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O..............................................................................0.05g
CaCl 2 ·2H 2 O ...............................................................................4.0mg
Cresol Red (0.0005% solution)................................................25.0mL
Ferric EDTA solution.................................................................0.1mL
pH 8.2–8.4 at 25°C
Ferric EDTA Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................0.5g
Disodium EDTA.........................................................................0.14g
H 2 SO 4 , concentrated................................................................0.05mL
Preparation of Ferric EDTA Solution: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add CaCl 2 ·2H 2 O and MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 500.0mL. Mix thorough-
ly. In a separate flask, add remaining components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Autoclave both
solutions separately for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically combine the two sterile solutions. Mix thoroughly. Asepti-
cally distribute into sterile tubes or flasks. After inoculation, maintain
pH at 8.2–8.4 with sterile 50% K 2 CO 3 solution.
Use:  For the cultivation and maintenance of Nitrosomonas europaea.