Nitrosomonas europaea Medium

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrosomonas europaea Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 .....................................................................................1.7g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.02g
K 2 HPO 4 .....................................................................................0.015g
Ferric EDTA...............................................................................1.0mg
Trace elements solution .............................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Trace Elements Solution:
Composition per 100.0mL:
MnCl 2 ·4H 2 O................................................................................0.02g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.01g
ZnSO 4 ·7H 2 O...............................................................................0.01g
CuSO 4 ·5H 2 O..............................................................................2.0mg
CoCl 2 ·6H 2 O...............................................................................0.2mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5 with
K 2 CO 3 . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. After inoculation, maintain pH at 7.5–7.8 with sterile
50% K 2 CO 3 solution.
Use:  For the cultivation and maintenance of Nitrosomonas europaea.