Nitrosococcus oceanus Medium

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrosococcus oceanus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Phenol Red....................................................................................5.0g
NH 4 ·Cl ......................................................................................0.635g
MgSO 4 ·7H 2 O............................................................................0.357g
K 2 HPO 4 ....................................................................................43.0mg
CaCl 2 ·H 2 O ...............................................................................20.0mg
Chelated metals solution............................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Chelated Metals Solution:
Composition per liter:
EDTA............................................................................................6.0g
FeCl 3 ·6H 2 O...................................................................................1.0g
MnSO 4 ·H 2 O..................................................................................0.6g
ZnSO 4 ·7H 2 O.................................................................................0.3g
Na 2 MoO 4 ·2H 2 O..........................................................................0.15g
CoCl 2 ·6H 2 O...............................................................................4.0mg
CuSO 4 ·5H 2 O..............................................................................4.0mg
Preparation of Chelated Metals Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except chelated metals
solution, to filtered seawater and bring volume to 1.0L. Mix thorough-
ly. Adjust pH to 7.5 with sterile 0.1M K 2 CO 3 . Autoclave for 15 min at
15 psi pressure–121°C. Aseptically add 1.0mL of sterile chelated met-
als solution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of Nitrosococcus oceanus.