Nitrosococcus Medium

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrosococcus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 ..................................................................................1.32g
MgSO 4 ·7H 2 O..............................................................................0.38g
CaCl 2 ·2H 2 O ................................................................................0.02g
K 2 HPO 4 ......................................................................................8.7mg
Chelated iron..............................................................................1.0mg
MnCl 2 · 4H 2 O ..............................................................................0.2mg
Na 2 MoO 4 ·2H 2 O.........................................................................0.1mg
ZnSO 4 ·7H 2 O..............................................................................0.1mg
CoCl 2 ·6H 2 O................................................................................2.0μg
Phenol Red (0.04% solution)...................................................3.25mL
pH 7.5–7.8 at 25°C
Preparation of Medium: Add components to filtered seawater and
bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5–7.8 with 1N
HCl. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–
121°C.
Use:  For the cultivation of Nitrosococcus oceanus.