Nitrogen-Free Mineral Agar for Derxia

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrogen-Free Mineral Agar for Derxia

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Glucose .......................................................................................10.0g
K 2 HPO 4 ........................................................................................0.5g
MgSO 4 ·7H 2 O..............................................................................0.25g
NaCl............................................................................................0.25g
CaCl 2 .............................................................................................0.1g
FeSO 4 ·7H 2 O .................................................................................0.1g
Na 2 MoO 4 ·2H 2 O.........................................................................5.0mg
pH 6.9 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.9.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use:  For the cultivation and maintenance of Derxia gummosa.