Nitrococcus Medium

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrococcus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1004.0mL:

NaNO 2  solution..........................................................................1.0mL
K 2 HPO 4  solution........................................................................1.0mL
NaHCO 3  solution .......................................................................1.0mL
Chelated metals solution............................................................1.0mL
pH 7.5 ± 0.1 at 25°C
NaNO 2  Solution:
Composition per 100.0mL:
NaNO 2 .........................................................................................10.0g
Preparation of NaNO 2  Solution:  Add NaNO 2  to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
K 2 HPO 4  Solution:
Composition per 100.0mL:
K 2 HPO 4 .........................................................................................2.5g
Preparation of  K 2 HPO 4  Solution:  Add K 2 HPO 4  to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
NaHCO 3  Solution:
Composition per 100.0mL:
NaHCO 3  .......................................................................................5.0g
Preparation of  NaHCO 3  Solution:  Add NaHCO 3  to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Chelated Metals Solution:
Composition per liter:
EDTA............................................................................................6.0g
FeCl 3 ·6H 2 O...................................................................................1.0g
MnSO 4 ·H 2 O..................................................................................0.6g
ZnSO 4 ·7H 2 O.................................................................................0.3g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.15g
CoCl 2 ·6H 2 O ...............................................................................4.0mg
CuSO 4 ·5H 2 O..............................................................................4.0mg
Preparation of Chelated Metals Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Adjust pH of 1.0L of seawater to pH 7.5
with NaOH. Add 1.0mL of chelated metals solution to the seawater.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°C. Aseptically add 1.0mL each of sterile NaNO 2 , K 2 HPO 4 , and
NaHCO 3  solutions. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use: For the cultivation of Nitrococcus species.