Nitrobacter Medium B

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrobacter Medium B

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaNO 2 ..........................................................................................1.0g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ..........................................................................................0.5g
NaCl..............................................................................................0.3g
Fe 2 (SO 4 ) 3 ...................................................................................5.0mg
MnSO 4 .......................................................................................2.0mg
Marble chips.........................................................................as needed
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components, except marble chips, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Wash
marble chips in distilled/deionized water. Put a few chips into test
tubes. Autoclave for 60 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically distribute cooled sterile medium into test tubes to cover
marble chips.
Use:  For the cultivation of Nitrobacter species.