Nitrobacter Medium 203

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrobacter Medium 203

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution C..................................................................................1.0mL
Solution A..................................................................................0.5mL
Solution B..................................................................................0.5mL
Solution D..................................................................................0.5mL
Solution E ..................................................................................0.5mL
Solution F...................................................................................0.2mL
Solution A:
Composition per 100.0mL:
CaCl 2 .............................................................................................2.0g
Preparation of Solution A: Add CaCl 2 to distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly.
Solution B:
Composition per 100.0mL:
MgSO 4 ·7H 2 O..............................................................................20.0g
Preparation of Solution B: Add MgSO 4 ·7H 2 O to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Solution C:
Composition per 100.0mL:
Chelated iron (Sequestrene)..........................................................0.1g
Preparation of Solution C: Add chelated iron to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Solution D:
Composition per liter:
MnCl 2 ·4H 2 O..................................................................................0.2g
Na 2 MoO 4 ·2H 2 O ............................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
CuSO 4 ·5H 2 O...............................................................................0.02g
CoCl 2 ·6H 2 O ...............................................................................2.0mg
Preparation of Solution D: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Solution E:
Composition per 100.0mL:
NaNO 2 .........................................................................................41.4g
Preparation of Solution E: Add NaNO 2 to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly.
Solution F:
Composition per 100.0mL:
K 2 HPO 4 .......................................................................................1.74g
Preparation of Solution F: Add K 2 HPO 4 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add the appropriate volumes of solutions
A–F to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C.
Use:  For the cultivation and maintenance of Nitrobacter species and
Nitrobacter winogradskyi.