Nitrobacter agilis Medium

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrobacter agilis Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

CaCO 3 .........................................................................................10.0g
NaCl..............................................................................................0.3g
Na 2 CO 3  .......................................................................................0.25g
KNO 2 ..........................................................................................0.17g
K 2 HPO 4 .......................................................................................0.14g
MgSO 4 ·7H 2 O..............................................................................0.14g
FeSO 4 ·7H 2 O................................................................................0.03g
MnSO 4 ·4H 2 O..............................................................................0.01g
Biotin solution .........................................................................10.0mL
Biotin Solution:
Composition per 10.0mL:
Biotin..........................................................................................0.15g
Preparation of Biotin Solution: Add biotin to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add Na 2 CO 3  to distilled/deionized water
and bring volume to 200.0mL. Mix thoroughly. In a separate flask, add
the remaining components, except the biotin solution, to distilled/de-
ionized water and bring volume to 790.0mL. Autoclave the Na 2 CO 3
solution and salts solution separately for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Aseptically combine the sterile Na 2 CO 3  solu-
tion, sterile salts solution, and sterile biotin solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Nitrobacter agilis.