Nitrilotriacetate Medium

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrilotriacetate Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1002.0mL:

MgSO 4 ·7H 2 O................................................................................1.0g
Nitrilotriacetate.............................................................................1.0g
Na 2 HPO 4 ·2H 2 O...........................................................................0.41g
KH 2 PO 4 .......................................................................................0.26g
CaCl 2 ·2H 2 O...................................................................................0.2g
Trace elements solution .............................................................1.0mL
Vitamin solution.........................................................................1.0mL
pH 6.5 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O ...........................................................................120.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................68.0mg
H 3 BO 3 ......................................................................................62.0mg
Na 2 MoO 4 ·2H 2 O ...................................................................... 24.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
CuCl 2 ·2H 2 O .............................................................................17.0mg
HCl (0.05M solution)....................................................................1.0L
Preparation of Trace Elements Solution : Add FeCl 2 ·4H 2 O to
1.0L of HCl solution. Mix thoroughly. Add distilled/deionized water
and bring volume to 1.0L. Add remaining components. Mix thorough-
ly.
Vitamin Solution:
Composition per liter:
Folic acid.....................................................................................20.0g
α-Lipoic acid............................................................................50.0mg
p-Aminobenzoic acid...............................................................50.0mg
Pantothenic acid.......................................................................50.0mg
Riboflavin ................................................................................50.0mg
Thamine·HCl............................................................................50.0mg
Vitamin B 12 ..............................................................................50.0mg
Nicotinamide............................................................................25.0mg
Biotin .......................................................................................20.0mg
Nicotinic acid...........................................................................20.0mg
Pyridoxamine·HCl ...................................................................10.0mg
Preparation of Vitamin Solution : Add components to distilled/
deionized water and bring volume to 1.0L. Stir for 2-3 hr. Filter steril-
ize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Chelatobacter heintzii and Chelatococcus
saccharophobus.