Nitriliruptor alkaliphilus Medium (DSMZ Medium 1105)

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nitriliruptor alkaliphilus Medium
(DSMZ Medium 1105)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 CO 3 .......................................................................................22.0g
Na 2 HCO 3 ......................................................................................8.0g
NaCl..............................................................................................6.0g
K 2 HPO 4 .........................................................................................0.5g
Isobutyroamide solution..........................................................10.0mL
Trace elements solution.............................................................1.0mL
Magnesium sulfate solution.......................................................1.0mL
Vitamin solution.........................................................................1.0mL
Thiosulfate solution.................................................................0.01mL
pH 9.5 ± 0.5 at 25°C
Isobutyroamide Solution:
Composition per 10.0ml:
Isobutyroamide...........................................................................0.87g
Preparation of Isobutyroamide Solution:  Add isobutyroamide
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Vitamin Solution:
Composition per 10.0ml:
Vitamin B 12 ................................................................................1.0mg
Preparation of Vitamin Solution:  Add vitamin B 12 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Magnesium Sulfate Solution:
Composition per 10.0mL:
MgSO 4 · 7H 2 O................................................................................2.0g
Preparation of Magnesium Sulfate Solution:  Add MgSO 4 · 7H 2 O
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature.
Thiosulfate Solution:
Composition per 10.0mL:
Na 2 S 2 O 3 ·5H 2 O..............................................................................1.6g
Preparation of Thiosulfate Solution:  Add Na 2 S 2 O 3 ·5H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N 2 . Adjust to pH 10.0. Filter sterilize.
Trace Elements Solution:
Composition per liter:
EDTA............................................................................................5.0g
FeSO 4 ·7H 2 O..................................................................................2.0g
H 3 BO 3 .........................................................................................0.01g
ZnSO 4 ·7H 2 O.................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
MnCl 2 ·4H 2 O................................................................................0.03g
NiCl 2 ·2H 2 O.................................................................................0.02g
NaMoO 4 ·2H 2 O............................................................................0.02g
CuCl 2 ...........................................................................................0.01g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3-4.
Preparation of Medium: Add components, except vitamin, trace
elements, thiosulfate, isobutyroamide, and magnesium sulfate solu-
tions, to distilled/deionized water and bring volume to 987.0mL. Mix
thoroughly. Dispense into closed bottles. Autoclave for 15 min at 15
psi pressure–121°C. Cool to room temperature. After cooling, there
may be some precipitate on the bottom. Decant into sterile bottle to
eliminate precipitate. Aseptically add vitamin, trace elements, thiosul-
fate, isobutyroamide, and magnesium sulfate solutions. Adjust pH to
9.5. Aseptically dispense into culture vessels.
Use: For the cultivation of Nitriliruptor alkaliphilus.