Nitrate Reduction Broth for Pseudomonas and Related Genera

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrate Reduction Broth for
Pseudomonas and Related Genera

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Beef extract...................................................................................1.0g
NaNO 3 ..........................................................................................0.1g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into test
tubes that contain an inverted Durham tube. Autoclave for 15 min at 15
psi pressure–121°C.
Use:  For the differentiation of members of the Pseudomonadaceae
based on their ability to reduce nitrate to nitrite or form N 2 gas. Test for
nitrates with sulfanilic acid and α-naphthylamine reagents. Bacteria
that reduce nitrate to nitrite turn the reagents red or pink.