Nitrate Reduction Broth, Clark

[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrate Reduction Broth, Clark

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .......................................................................................20.0g
KNO 3 or NaNO 3 ...........................................................................2.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into test
tubes that contain an inverted Durham tube. Autoclave for 15 min at 15
psi pressure–121°C.
Use:  For the differentiation of a variety of Gram-negative bacteria
based on their ability to reduce nitrate to nitrite or form N 2 gas. Test for
nitrates with sulfanilic acid and α-naphthylamine reagents. Bacteria
that reduce nitrate to nitrite turn the reagents red or pink.