MRS Medium, Modified

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MRS Medium, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
Beef extract...................................................................................5.0g
Sodium acetate·3H 2 O....................................................................5.0g
Yeast extract..................................................................................5.0g
K 2 HPO 4 ·3H 2 O ..............................................................................2.6g
Ammonium citrate........................................................................2.0g
Tween™ 80...................................................................................1.0g
L -Cysteine·HCl·H 2 O.....................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.1g
MnSO 4 ·4H 2 O...........................................................................50.0mg
Carbohydrate solution............................................................100.0mL
pH 6.3 ± 0.2 at 25°C
Carbohydrate Solution:
Composition per 100.0mL:
Fructose.........................................................................................7.0g
Glucose.........................................................................................7.0g
Maltose .........................................................................................7.0g
Sodium gluconate .........................................................................2.0g
Preparation of Carbohydrate Solution:  Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.3. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Asep-
tically add 100.0mL of sterile carbohydrate solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Lactobacillus pontis.