MRS Medium (DSMZ Medium 11)

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MRS Medium
(DSMZ Medium 11)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................20.0g
Casein peptone, tryptic digest.....................................................10.0g
Meat extract................................................................................10.0g
Yeast extract..................................................................................5.0g
Na-acetate.....................................................................................5.0g
K 2 HPO 4 .........................................................................................2.0g
(NH 4 ) 2 citrate................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·2H 2 O..............................................................................0.05g
Tween™ 80................................................................................1.0mL
pH 6.2–6.5
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.2 –
6.5. Gently heat and bring to boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Lactobacillus spp., Leu-
conostoc mesenteroides, and Pediococcus pentosaceus.