MRS Agar with 0.5% Cysteine (LMG Medium 131)

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MRS Agar with 0.5% Cysteine
(LMG Medium 131)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................20.0g
Agar............................................................................................12.0g
Peptone .......................................................................................10.0g
Beef extract...................................................................................5.0g
Sodium acetate·3H 2 O....................................................................5.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
Diammonium hydrogen citrate.....................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·4H 2 O...........................................................................38.0mg
L -Cysteine·HCl solution ........................................................100.0mL
Tween™ 80................................................................................1.0mL
pH 6.5 ± 0.2 at 25°C
L -Cysteine · HCl Solution:
Composition per 100.0mL:
L -Cysteine·HCl .............................................................................5.0g
Preparation of  L -Cysteine · HCl Solution: Add 5.0g of  L -cyste-
ine·HCl to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except  L -cysteine·HCl
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Adjust pH to 6.5. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add 100.0mL of sterile  L -
cysteine·HCl solution. Mix thoroughly. Aseptically pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Lactobacillus ruminis and
Lactobacillus vitulinus.