MRS Agar with Cysteine (LMG Medium 118)

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MRS Agar with Cysteine
(LMG Medium 118)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................20.0g
Peptone .......................................................................................10.0g
Agar............................................................................................12.0g
Beef extract...................................................................................5.0g
Sodium acetate·3H 2 O....................................................................5.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
Diammonium hydrogen citrate.....................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·4H 2 O...........................................................................38.0mg
L -Cysteine solution..................................................................10.0mL
Tween™ 80................................................................................1.0mL
pH 6.5 ± 0.2 at 25°C
L -Cysteine Solution:
Composition per 10.0mL:
L -Cysteine.....................................................................................0.5g
Preparation of  L -Cysteine Solution: Add 0.1g of  L -cysteine to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except  L -cysteine solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Adjust pH to 6.5. Gently heat and bring to boiling. Distrib-
ute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°C. Aseptically add 10.0mL of sterile  L -cysteine solution.
Mix thoroughly. Aseptically pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the isolation and cultivation of Leuconostoc mesenteroides
and other lactic acid bacteria.