Motility Test and Maintenance Medium

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]

Motility Test and Maintenance Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..............................................................................................9.0g
Tryptose ........................................................................................8.0g
NaCl..............................................................................................5.0g
Pancreatic digest of gelatin...........................................................2.5g
Beef extract...................................................................................1.5g
pH 7.2 ± 0.1 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes in 7.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C. Pass the cooled tubes
into an anaerobic chamber containing 85% N 2 + 10% H 2 + 5% CO 2 .
Use: For the cultivation, maintenance, and observation of the motility
in a variety of anaerobic bacteria.