Mobiluncus Agar (LMG Medium 117)

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Mobiluncus Agar
(LMG Medium 117)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Special peptone...........................................................................23.0g
Agar No. 1...................................................................................10.0g
Starch, soluble.............................................................................10.0g
NaCl..............................................................................................5.0g
Resazurin ..................................................................................10.0μg
Rabbit serum............................................................................20.0mL
pH 7.1–7.5
Source:  Special peptone and Agar No. 1 are available from Oxoid
Unipath.
Preparation of Medium: Add components, except rabbit serum, to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL sterile
rabbit serum. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the cultivation of Mobiluncus curtisii subsp. holmesii and
Mobiluncus mulieris.