MN Marine Medium with Vitamin B 12

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

MN Marine Medium with Vitamin B 12

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Noble agar...................................................................................10.0g
NaNO 3 .........................................................................................0.75g
MgSO 4 ·7H 2 O..............................................................................0.04g
CaCl 2 ·2H 2 O.................................................................................0.02g
K 2 HPO 4 ·3H 2 O.............................................................................0.02g
Na 2 CO 3  .......................................................................................0.02g
Citric acid...................................................................................3.0mg
Ferric ammonium citrate............................................................3.0mg
Disodium potassium EDTA.......................................................0.5mg
Vitamin B 12 ...............................................................................20.0μg
Trace metal mix A-5..................................................................1.0mL
pH 8.5 ± 0.2 at 25°C
A-5 Trace Metal Mix:
Composition per liter:
H 3 BO 3 .........................................................................................2.86g
MnCl 2 ·4H 2 O................................................................................1.81g
ZnSO 4 ·7H 2 O.............................................................................0.222g
CuSO 4 ·5H 2 O.............................................................................0.079g
Na 2 MoO 4 ·2H 2 O ........................................................................0.039g
Co(NO 3 ) 2 ·6H 2 O ........................................................................0.049g
Preparation of A-5 Trace Metal Mix: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to 750.0mL of seawater
and bring volume to 1.0L with glass-distilled water. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. After autoclaving, adjust pH to 8.5 with KOH.
Use:  For the cultivation and maintenance of Dermocarpa species,
Dermocarpella species, Myxosarcina species, Phormidium species,
Pleurocapsa species, Synechococcus species, Synechocystis species,
and Xenococcus species.