Alcaligenes Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alcaligenes Medium
Composition per liter:
Tris..............................................................................................6.06g
NaCl............................................................................................4.68g
KCl..............................................................................................1.49g
NH 4 Cl .........................................................................................1.07g
Na 2 SO 4 ........................................................................................0.43g
Na 2 HPO 4 ·12H 2 O.........................................................................0.23g
MgCl 2 ·6H 2 O..................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.03g
Ferric ammonium citrate...........................................................0.005g
Sodium succinate solution.......................................................10.0mL
CuSO 4 solution ..........................................................................2.5mL
Trace elements solution SL-7 ....................................................1.0mL
Sodium Succinate Solution:
Composition per 100.0mL:
Sodium succinate........................................................................40.0g
Preparation of Sodium Succinate Solution:  Add sodium succi-
nate to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
CuSO 4 Solution:
Composition per 100.0mL:
CuSO 4 .........................................................................................16.0g
Preparation of CuSO 4 Solution:  Add CuSO 4 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Trace Elements Solution SL-7:
Composition per 1001.0mL:
CoCl 2 ·6H 2 O...........................................................................200.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
H 3 BO 3 ......................................................................................60.0mg
Na 2 MoO 4 ·2H 2 O.......................................................................40.0mg
CuCl 2 ·2H 2 O.............................................................................20.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
HCl (25%)..................................................................................1.0mL
Preparation of Trace Elements Solution SL-7:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except CuSO 4 solution
and sodium succinate solution, to distilled/deionized water and bring
volume to 987.5mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 10.0mL of sterile CuSO 4  solution and
2.5mL of sterile sodium succinate solution. Mix thoroughly. Aseptical-
ly distribute into sterile tubes or flasks.
Use: For the cultivation of Alcaligenes species.