Alcaligenes Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alcaligenes Medium
Composition per liter:
Peptone..........................................................................................5.0g
Beef extract...................................................................................3.0g
Ferric citrate..................................................................................0.2g
Ammonium lactate solution.......................................................3.0mL
pH 7.0 ± 0.2 at 25°C
Ammonium Lactate Solution:
Composition per 100.0mL:
Lactic acid...................................................................................60.0g
Preparation of Ammonium Lactate Solution: Dissolve lactic
acid in 100.0mL of distilled/deionized water. Neutralize with NH 4 OH
to pH 7.0.
Preparation of Medium: Add peptone, beef extract, and ammoni-
um lactate to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Add ferric citrate aseptically. Mix thoroughly.
Aseptically distribute into tubes or flasks.
Use: For the cultivation of Alcaligenes tolerans.