Mineral Salts Medium

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Salts Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .......................................................................................4.0g
KH 2 PO 4 .........................................................................................1.5g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Ferric ammonium citrate............................................................5.0mg
Modified Hoagland trace elements solution..............................1.0mL
pH 7.0 ± 0.2 at 25°C
Modified Hoagland Trace Elements Solution:
Composition  per 3.6L:
H 3 BO 3 ......................................................................................... 11.0g
MnCl 2 ·4H 2 O .................................................................................7.0g
AlCl 3 .............................................................................................1.0g
CoCl 2 ............................................................................................1.0g
CuCl 2 ............................................................................................1.0g
KI..................................................................................................1.0g
NiCl 2 .............................................................................................1.0g
ZnCl 2 .............................................................................................1.0g
BaCl 2 .............................................................................................0.5g
KBr ...............................................................................................0.5g
LiCl...............................................................................................0.5g
Na 2 MoO 4 ......................................................................................0.5g
SeCl 4 .............................................................................................0.5g
SnCl 2 ·2H 2 O...................................................................................0.5g
NaVO 3 ·H 2 O..................................................................................0.1g
Preparation of Modified Hoagland Trace Elements Solu-
tion: Prepare each component as a separate solution. Dissolve each
salt in approximately 100.0mL of distilled/deionized water. Adjust the
pH of each solution to below 7.0. Combine all the salt solutions and
bring the volume to 3.6L with distilled/deionized water. Adjust the pH
to 3–4. A yellow precipitate may form after mixing. After a few days,
it will turn into a fine white precipitate. Mix the solution thoroughly be-

fore using.

Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Rhodococcus rhodo-
chrous.