AKI Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

AKI Medium
Composition per liter:
Peptone .......................................................................................15.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................4.0g
Sodium bicarbonate solution...................................................30.0mL
pH 7.2 ± 0.2 at 25°C
Sodium Bicarbonate Solution:
Composition per 100.0mL:
NaHCO 3 ......................................................................................10.0g
Preparation of Sodium Bicarbonate Solution: Add sodium bi-
carbonate to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize. Use freshly prepared solution.
Preparation of Medium: Add components, except sodium bicarbon-
ate solution, to distilled/deionized water and bring volume to 970.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile sodium bicarbonate solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks. Prepare medium
freshly.
Use: For the cultivation of Vibrio cholerae and other Vibrio species.
Albumin Fatty Acid Broth, Leptospira Medium
See: Bovine Albumin Tween™ 80 Medium, Ellinghausen
and McCullough, Modified
Albumin Fatty Acid Semisolid Medium, Modified
See: Bovine Albumin Tween™ 80
Semisolid Medium, Ellinghausen and McCullough, Modi-
fied