AK Agar No. 2 (Sporulating Agar)

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

AK Agar No. 2
(Sporulating Agar)
Composition per liter:
Agar ............................................................................................15.0g
Pancreatic digest of gelatin...........................................................6.0g
Pancreatic digest of casein............................................................4.0g
Yeast extract..................................................................................3.0g
Beef extract...................................................................................1.5g
Glucose.........................................................................................1.0g
MnSO 4 ·7H 2 O................................................................................0.3g
pH 6.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Distribute into tubes or flasks. Autoclave
for 20 min at 15 psi pressure–121°C. Make sure medium is dissolved
before autoclaving.
Use: For the preparation of spore suspensions used to detect antibiotic
residues in milk and dairy products.