Microcyclus major Medium

[所属分类:培养基配方] [发布时间:2021-5-28] [发布人:网站管理员2] [阅读次数:] [返回]

Microcyclus major Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .........................................................................................1.0g
Peptone..........................................................................................1.0g
KNO 3 ............................................................................................0.1g
K 2 HPO 4 .......................................................................................0.07g
MgSO 4 ·7H 2 O..............................................................................0.03g
Trace elements solution .............................................................1.0mL
Trace Elements Solution:
Composition per liter:
Disodium EDTA .........................................................................10.0g
FeSO 4 ·7H 2 0...................................................................................9.3g
NaBO 3 ·4H 2 O.................................................................................2.6g
MnCl 2 ·4H 2 O..................................................................................1.8g
CaCl 2 .............................................................................................1.2g
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O....................................................................1.0g
ZnSO 4 ·7H 2 O.................................................................................0.2g
CuSO 4 ·5H 2 O...............................................................................0.08g
Co(NO 3 ) 2 ·H 2 O ............................................................................0.02g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Microcyclus major.