M-FC Agar Base

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

M-FC Agar Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Lactose........................................................................................12.5g
Tryptose ......................................................................................10.0g
Proteose peptone...........................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
Bile salts mixture..........................................................................1.5g
Aniline Blue..................................................................................0.1g
Rosolic acid solution................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Rosolic Acid Solution:
Composition per 10.0mL:
Rosolic acid...................................................................................0.1g
Preparation of Rosolic Acid Solution:  Add rosolic acidto dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat to dis-
solve components. Do not autoclave. Cool to 50°C. Pour into Petri
dishes or aseptically distribute into sterile tubes.
Use: For the detection and enumeration of fecal coliforms using the
membrane filter technique at 44.5°C.